پرینت

illumina

High Performance Real Time PCR System icludes:

Full-Featured Open Platform:

Delivers high-precision (< 0.167 SD Cq)
Provides sensitive fluorescence detection down to a single copy
Delivers ±0.1°C well-to-well uniformity
Supports most popular real-time PCR chemistries (calibrated for SYBR, FAM, HEX, VIC, ROX, and Cy5 dyes

Real-time applications include:

   Absolute quantification by standard curve

Relative quantification using ΔΔCq method with support for multiple reference gene normalization

   Allelic discrimination by end-point fluorescence

SNP genotyping, mutation screening and methylation analysis by High-Resolution Melt (HRM) curve analysis

Specification:

Thermal system                             Single Peltier-based system

Block format                                    48-well block

Consumables                                   48-well Eco plates and optical Eco adhesive seals

Accessories                                      Eco sample loading dock

Sample volumes                           5–20µl validated (optimized for standard 20µl protocols)

Average block ramp rate                             5.5°C/sec

Temperature range                                      35–100°C

Temperature uniformity                              ± 0.1°C

Optical system     

LED excitation (452–486 nm and 542–582 nm), four emission filters (505–545nm, 562–596 nm, 604–644 nm, and 665–705 nm) and CCD camera

Calibrated dyes at shipment             

  SYBR, FAM, HEX, ROX, Cy5: factory-calibrated. Additional dyes within wavelength range of Eco filters are supported with no additional calibration required for implementation

Data collection                  

Data collected in all four filters for all wells regardless of plate setup; plate setup for data analysis can be altered after run completion.

Real-Time PCR run time (40 cycles)                         Less than 40 minutes 

Electrical                                                                  Voltage: 120VAC=10%
Nominal current draw:                                            8A
Frequency:                                                             50/60 Hz=1%
                                                                            Peak Power 500VA, typical power is 180VA

Software   

Eco System Software supports all chemistries and a variety of applications, including absolute quantification, relative quantification, allelic discrimination, and high resolution melt curve analysis (HRM)        

Warranty                                                    12-month warranty (includes parts and labor)

 

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